摘要
[方法]采用富集培养法从受杂环类污染的土壤中分离喹啉降解菌株。[结果]分离得到1株以喹啉为唯一氮源、葡萄糖为碳源生长的QLD-9菌株,经16S rDNA鉴定为假单胞菌属(Pseudomonas sp.),该菌株能在48 h内将300 mg/L的喹啉降解99.9%以上,对600 mg/L以内喹啉具有良好的降解效果。[结论]QLD-9菌株对环境中喹啉的生物修复具有很好的应用潜力。
[Methods] Quinoline-degrading bacteria were isolated from contaminated soil by using enrichment culture method. [Results] A bacterial strain QLD-9 of utilizing quinoline as its sole nitrogen source and utilizing glucose as the carbon source was obtained. The strain was identified as Pseudomonas sp. according to the results of 16S rDNA sequence analysis. The strain could keep excellent degradation activity to quinoline with a concentration under 600 mg/L, and above 99.9% of quinoline with a concentration of 300 mg/L could be degraded within 48 h. [Conclusions] Strain QLD-9 showed potential of application for bio-remediation to quinoline in environment,
出处
《农药》
CAS
北大核心
2012年第6期424-426,共3页
Agrochemicals
基金
辽宁省自然科学基金(40971183)
关键词
喹啉
假单胞菌属
生物降解
quinoline
Pseudomonas sp.
biodegradation