四川省106株结核分枝杆菌spoligotyping基因分型研究

Genotyping of Mycobacterium Tuberculosis Isolates in Sichuan Province with Spoligotpying

目的了解四川省结核分枝杆菌基因型构成情况以及北京基因型菌株在四川省结核分枝杆菌中所占的比例,为四川省结核病预防控制提供分子流行病学依据。方法采用间隔区寡核苷酸分型(spacer oligonucletide typing,spoligotyping)方法对106株四川省结核分枝杆菌进行分型分析,将spoligotyping分型结果与SITVIT2数据库进行比较确定菌株的基因家族。结果根据spoligotyping分型结果及与数据库SITVIT2的比较,106株结核分枝杆菌表现为30种基因型,21种基因型为数据库中存在的基因型,9种基因型为新的基因型。基因家族分析发现,65株结核分枝杆菌为北京家族菌株(61.32%),26株为T家族菌株,4株为MANU家族菌株,U型及H型家族菌株各1株。9株新基因型的菌株没有进行基因家族的确定。结论本研究对四川省结核分枝杆菌的基因型构成以及北京家族菌株的流行程度做了初步的描述。对四川省结核病的流行趋势分析、结核病的预防控制等具有重要的意义。

Objective To study the population of mycobacterium tuberculosis and the prevalence of Beijing genotype strains in Si- chuan Province, and to provide the molecular epidemiological evidence for prevention and control of tuberculosis in Sichuan. Methods A total of 106 mycobacterium tuberculosis isolates from Sichuan province were genotyped by Spoligotyping and the Spoligotyping results were compared with the SITVIT2 database. Results The Spoligotyping results showed that thirty spoligotypes were identified： 21 spoligotypes had shared international type number in the database and the other 9 spoligotypes were novel. Family assignment revealed that the most frequent strains were Beijing family strains （61.32%） followed by T family （24.53%）, MANU family （3.37%）. In addition, the strains belonged to H family and U family were also found in Sichuan province. Conclusion This study provides preliminary insight into the population structure of mycobacterium tuberculosis circulating in Sichuan province as well as the prevalence of Beijing family strains in this area. It is important for the prevention and control of tuberculosis in Sichuan province.