微囊化人血管抑素基因工程细胞对人脐静脉内皮细胞增殖的影响

Effect of Microencapsulated Human Angiostatin Gene Engineered Cell on the Proliferation of Human Umbilical Vein Endothelial Cell

目的了解海藻酸钠-多聚赖氨酸-海藻酸钠(alginate-polylysine-alginate,APA)微囊化人血管抑素基因工程细胞对人脐静脉内皮细胞(Huecv304)增殖的影响。方法采用APA制备微囊包裹的表达人血管抑素(human angiostatin,hAS)的基因工程细胞(APA-hAS/293细胞微囊)或空载体转染的基因工程细胞(APA-0/293细胞微囊)。分别将上述两种细胞微囊以不同浓度与人脐静脉内皮细胞Huecv304细胞共培养,于培养0、24、48及72h时,以MTT法测定Huecv304细胞的增殖情况。结果 APA-hAS/293细胞微囊对共培养的Huecv304细胞的增殖具有明显的抑制作用(P<0.01),且具有良好的量效关系。而对照组APA-0/293细胞微囊对Huecv304细胞的增殖无明显抑制作用。结论体外培养的表达人血管抑素基因的工程细胞的分泌物可以通过微囊膜并对人脐静脉内皮细胞的增殖产生显著的抑制效应。

Objective To study the effect of microencapsulated human angiostatin gene engineered cell on the proliferation of human umbilical vein endothelial cell Huecv304. Methods Alginate - polylysine - alginate （APA） was used to encapsulate 293 cells expressing human angiostain （ APA - hAS/293 ） or empty vector （ APA - 0/293 ）. Microencapsulated APA - hAS/293 or APA - 0/293 cells were co - cultured with Huecv304 in different cell concentration. After 0, 24, 48 and 72h, the proliferation of Huecv304 was measured with MTT assay. Results Microeneapsulated APA- hAS/293 inhibited the proliferation of coeultured Hueev304 cells （P 〈 0. 01 ）in a dose - dependent manner. While microencapsnlated APA - 0/293 didn＇t inhibit the proliferation of Hueev304 cells in co - culture. Conclusion The secretion of gene engineered cells expressing human angiostatin could pass through the membrane of microencapsule and inhibit the proliferation of human umbilical vein endothelial cells.