血清GPC3联合AFP检测在HCC中的临床意义

Clinical Significance of Serum GPC3 and AFP in Patients with Hepatocellular Carcinoma

目的探讨血清磷脂酰肌醇蛋白聚糖3(GPC3)和甲胎蛋白(AFP)在肝细胞癌(HCC)患者中的临床意义。方法采用ELISA法检测血清GPC3和血清AFP,分析72例HCC患者、58例肝良性病变组和40例健康组血清GPC3和AFP水平。结果 HCC患者血清GPC3和AFP水平显著高于肝良性病变组和健康组(P<0.05);肝良性病变组和健康组中表达无统计学意义(P>0.05)。两者表达与肿瘤大小、是否侵袭和病理分级有关(P<0.05);年龄、性别、有无HbsAg感染和包膜与两者表达无关(P>0.05)。单项检测灵敏度、准确度较低;联合检测灵敏度达84.7%,准确度达90.1%;两者在HCC中表达未存在直接关联(P>0.05)。结论联合检测血清GPC3和AFP水平有助于HCC的早期临床诊断。

Objective To explore the value of serum glypican -3 （GPC3） and alphafetoprotein（AFP） in patiens with hepatocellular carcinoma（HCC）. Methods ELISA was employed to detect serum GPC3 levels. Serum AFP levels from patients serum with HCC （n =72） ,nonHCC tumor samples （n =58） and controls（n =40） were measured with chemilumiescense immuniassay. Results The levels of GPC3 and AFP were significantly higher in HCC than nonHCC tumor samples and controls （ P 〈 0.05 ）. The levels were not much different from those in nonHCC tumor samples and controls（P 〉 0.05）. The levels of GPC3 and AFP seemed to be correlated with tumor diameter, the stage （ by TNM classification） of HCC and/or distant metastasis （ P 〈 0.05）. With combined detection of GPC3 and AFP,the sensitivity would be 84.7% , accuracy would be 90.1%. Moreover, there was no correlation between the levels of GPC3 and AFP. Conclusion Detected the expression of GPC3 and AFP are usefulness for diagnosis of primary HCC.