咖啡因对慢性刺激过程中小鼠行为和学习记忆的影响

Effect of Caffeine on Learning and Memory Ability and Behavior of Mice in Chronic Stress

目的探讨咖啡因对慢性刺激小鼠行为和学习记忆的影响。方法选取28只C57BL/6J小鼠,随机分为正常组(n=10):空白+0.15ml生理盐水;刺激组(n=8):慢性刺激+0.15ml生理盐水;药物组(n=10):慢性刺激+15mg/kg咖啡因。刺激组和药物组分别孤养,并接受持续10周的慢性不可预知温和刺激。结合旷场实验和Morris水迷宫方法,评价小鼠的行为和学习记忆能力。结果 10周后刺激组和药物组的水平穿越格子数[(118.50±12.20)次,(80.10±16.02)次)]都明显高于正常组(49.00±10.05)次(P<0.01);水迷宫测试中,药物组定位航行训练第1天的潜伏期明显短于正常组和刺激组[(13.12±2.27)s,(27.34±5.00)s,(30.62±4.54)s](P<0.05);药物组和正常组体重增加量[(3.40±0.37)g,(5.40±0.69)g]有明显差异(P<0.05),10周后药物组和正常组体重[(24.60±0.43)g,(26.60±0.62)g]有明显差异(P<0.05)。结论长期慢性低剂量咖啡因注射可以减缓慢性刺激下小鼠体重增加,同时还能加强刺激状态下小鼠的空间学习记忆能力;长期慢性刺激也能加强小鼠的探索行为。

Objective To explore the effects of caffeine on learning and memory ability and behavior of mice in chronic stress. Methods Totally 28 mice were randomly divided into 3 group：Control group：vehicle ＋ 0.15mlsaline; Chronic stress group：chronic strsst ＋ 0. 15ml saline; Drug group：chronic stress ＋ 15mg/kg Caffeine. Chronic stress and drug group were received chronic unpredictable mild stress of ten weeks and single house. The learning and memory ability were measure by morris water maze and behavior was measured by openfield test. Results After 10 weeks,the activity in the open field test in both drug groups and chronic stress group [ （118.50 ± 12.20 ） counts, （80.10 ± 16.02 ） counts） ] increased more than control group （49.00 ±10.05 ） counts （ P 〈 0.0I ）. Compared to the control group and chronic stress group [ （ 27.34 ± 5.00 ） s, （ 30.62 ± 4.54 ） s ], the escape latency of drug group （ 13.12 ~ 2.27 ） in the morris water maze was decreased （ P 〈 0.05 ）. After 10 weeks, the weight of drug group was more than control group [ （24. 60± 0.43 ） g, （ 26.60 ± O. 62 ） g] （ P 〈 0.05 ）, The increase of weight of drug group was slower than control group [ （ 3.40 ± O. 37 ） g, （ 5.40 ± 0. 69 ） g ] （P 〈 0.05 ）. Conclusion Long period consumption of caffeine could slower the weight increase and improve the learning and memory after chronic stress. Long period chronic stress could also enhance the explore ability.