壳聚糖对棉花种子萌发及相关酶活性的影响

Effect of Chitosan on Seed Germination and Relevant Enzymes,Activity of Cotton

用0.1%、0.2%、0.3%、0.4%、0.5%五种浓度的壳聚糖溶液处理棉花种子12 h,以蒸馏水浸种12 h为对照。室温培养7 d,每天自然光照8 h,棉花种子的发芽率、发芽势、萌发指数、活力指数及α-淀粉酶活性、过氧化物酶活性和过氧化氢酶活性均比对照高,且随着壳聚糖浓度的增大各指标均呈先升高后降低的趋势,处理浓度为0.2%时,种子的发芽率、发芽势、萌发指数、活力指数及α-淀粉酶活性、过氧化物酶活性和过氧化氢酶活性分别提高了16.7%、3.17%、2.63、30.7、96.87%、37.42%、33.78%,效果最佳。

Cotton seeds were soaked in chitosan solution of different concentrations（0.1%,0.2%,0.3%,0.4%,0.5%）for 12 hours in comparison with seeds in water.Then cultured them for 7 days at room temperature and 8 hours at natural light every day.Seed germination rate,germination power,germination index,vigor index and activity of a-amylase,POD and CAT were higher than the control.With the increasing of chitosan concentration,all indexes increaded at first and then decreased,When the concentration was 0.2%,the seed germination rate,germination energy,germination index,vigor index,a-amylase,POD and CAT activity were increased by 16.7%,3.17%,2.63,30.7,96.87%,37.42% and 33.78% respectively,and it was the optimum concentration.