HPLC法测定紫果西番莲叶中白杨素的含量

Content Determination of Chrysin from the Leaves of Passiflora edulis by HPLC

目的:建立测定紫果西番莲叶中白杨素含量的方法。方法:样品经硅胶柱色谱分离纯化后进行高效液相色谱分析。色谱柱为HypersilODSC18(250mm×4.6mm,5μm),流动相为乙腈-0.1磷酸(18:82),流速为1.0mL.min-1,检测波长为220nm,进样量为10μL。结果:白杨素进样浓度在0.012～0.120mg.mL-1范围内与峰面积积分值呈良好线性关系(r=0.9980);平均加样回收率为99.41%,RSD=1.6%(n=6)。结论:该方法准确、可靠、重复性好,可用于西番莲属植物中白杨素含量的测定。

OBJECTIVE： To establish the method for the content determination of chrysin in the leaves of Passiflora edulis. METHODS： Samples were separated and purified by using silica gel column chromatography and subjected to HPLC analysis. The separation was carried out on Hypersil ODS C18（250 mm×4.6 mm, 5 μm） column with acetonitrile-0.1% phosphoric acid （18：82） as mobile phase at the flow rate of 1.0 mL-min^-1. The UV detection wavelength was set at 220 mn and the injection volume was 10 μL. RESULTS： The linear range of chrysin was 0.012-0.120 mg.mL-1（r=0.998 0）with an average recovery of 99.41% （RSD 1.6%, n=6）. CONCLUSION： The method is accurate, reliable and reproducible, and it is can be used for the content determination of chrysin in leaves of P. edulis.