5'-磷酸二酯酶的制备及其在酵母抽提物生产中的应用

Making of 5'-phosphodicsterase and its appcication in producing yeast extract

本文着重论述了从工业废料———麦芽根中提取 5’———磷酸二酯酶的方法 ,通过正交试验认为其最佳提取工艺为 :麦芽根加七倍水量 ,在 4℃下浸提 2 0hr,可得到活力 1 40 μ/ml的 5’—磷酸二酯酶 ,将上述 5’—磷酸二酯酶加入经煮沸并冷却至 70℃ ,pH5 2— 5 4的酵母悬浮液中作用3hr,能显著提高抽提物中 (I +G)含量 ,增加产品鲜味。

This article discusses the extracting method of 5'-phosphodicsterase from the industrial uaste-mact root,and determines by orthogondl experiment.the best extracting condition:adding water which is seven times the amount of the mact root to the malt root for 20 hours at 4℃ we can obtain 5'-phosphodicsterare of 140 μ/ml.Adding the above 5'-phosphodicsterase into 10% yeast solution (pH5 2-5 4)which boies and cooled to 70℃ and interacting for 3hours. We can remarkably increase the content of I+G in the extracting solution. increase its freshness,and reduce the production cost.This is a econoncical and feasible method.