TopoⅡ及Ki67在基底细胞样乳腺癌中的表达及意义

Expression and clinical significance of ToPo Ⅱ and Ki67 Protein in Basal-like breast carcinoma

目的研究Ki67和TopoⅡ蛋白在基底细胞样乳腺癌中的表达意义。方法收集我院收治的浸润性乳腺癌标本，筛选出基底细胞样型乳腺癌（BLBC组）及83例非基底细胞样乳腺癌（非BLBC组）。用免疫组化S-P法染色检测TopoⅡ及Ki67在不同组织中的表达。结果Ki67蛋白在四种乳腺组织的阳性表达率差异有极显著性（X^2=18．36，（P=0．001），非BLBC组与BLBC组的Ki67蛋白表达差异有显著性，BLBC组的Ki67蛋白表达率水平最高。TopoⅡ表达与临床病理分期有密切关系，在BLBC组中肿瘤组织学分级越高，TopoⅡ蛋白表达越强（P〈0．05l非BLBC组中TopoII在Ⅱ级中阳性表达率最高。进一步进行Spearman等级相关分析，r=0．346，P=0．004，说明两种蛋白表达存在显著正相关性。结论Ki67蛋白在BLBC组、非BLBC组中的表达与组织学分级存在密切关系。TopoⅡ蛋白在BLBC组、非BLBC组中的表达与组织学分级、TNM分期存在密切关系。Ki67与TopoⅡ蛋白在BLBC组中表达显著高于非BLBC组。BLBC组中的Ki67与TopoⅡ蛋白表达存在着显著的正相关性。

Objective To study the expression and clinical significance of Ki67 protein and TOPOⅡ protein in basal-like breast carcinoma （BLBC）. Methods Invasive breast cancer specimens were selected in the Maternal and Child Service Center. 22 cases were selected as BLBC group. And 83 cases of non-BLBC （NBLBC） were randomly selected as NBLBC group. All of samples used SP immunohistochemical method to detect the express of Topo-Ⅱ and Ki67 in different groups. Results The Ki67 protein＇ s positive expression rate in four breast tissue was （ X^2=18.36, P=-0.001 ）, NBLBC group＇ s Ki67 protein had statistically difference with the BLBC group, showing that the BLBC group Ki67 protein expression rate level was the highest. Topo Ⅱ expression had close correlation with clinical pathologic stage. The result showed that in BLBC group, the higher the tumor histological grade, the higher the Topo Ⅱ protein expression（ P〈 0.05 and Topo Ⅱ positive rate of NBLBC group expressed highest in level Ⅱ. Further with Spearman rank correlation analysis （r =0.346, P=-0.004） showed significant positive correlation of the two proteins. Conclusion There was close relationship between Ki67 protein expression and histologic classification in BLBC and NBLBC groups. Topo Ⅱ expressed close correlation with histologic classification and TNM classification in BLBC and NBLBC groups. The express of Ki67 and Topo Ⅱ in BLBC is significantly higher than those in NBLBC. The express of Ki67 and Topo Ⅱ have positive correlation in BLBC.