短时受精精卵共孵育时间对受精率的影响

Effects of duration of sperm and egg co-incubation on the fertility rate in short-time insemination

目的探讨短时受精精卵共孵育时间对受精率的影响。方法170例行体外受精-胚胎移植（IVF—ET）患者常规促排卵，依据精卵共孵育时间分为短时受精组（A组：57例，精卵共孵育2～3h；B组：58例，精卵共孵育4～5h）和常规受精组（C组：55例，精卵共孵育18h）；之后将卵子转移至新鲜培养基中。比较各组的受精率、卵裂率、优胚率、临床妊娠率、胚胎种植率。结果三组受精率分别为63．01％、71．43％和78．72％，各组间的差异有统计学意义（P〈0．05）；C组的卵裂率、优胚率均低于A组和B组；C组与A组的卵裂率[97．05％vs99．25％]、C组与B组的优胚率[37．14％vs44．42％]之间的差异有统计学意义（P〈0．05）；各组间临床妊娠率、胚胎种植率差异无统计学意义（P〉0．05）。结论短时受精有助于提高卵裂率、优胚率，精印共孵育时间4～5h的受精率较2～3h高。

Objective To investigate effects of duration of sperm and egg co-incubation on the fertility rate in short time insemination. Methods 170 women undergoing in vitro fertilization - embryo transfer （ IVF-ET ） were divided into short-time insemination group （ group h 57 women, co-incubation for 2 h to 3 h; group 2：58 women, incubation for 4 h to 5 h ）and routine insemination group （ group 3： 55 women, co-incubation for 18 h ）based on duration of sperm and egg co-incubation. Then the oocytes were transferred to the fresh medium. The rates of fertility, cleavage, high-quality embryo, pregnancy, and implantation were compared among the groups. Results The fertility rate was 63.01%, 71.43%, and 78.72% in each group, with a significant difference （ P〈 0.05 ）. The rates of cleavage and high-quality embryo were lower in group 3 than in groups 1 and 2. There were significant differences in the cleavage rate between group 3 and group 1 （ 97.05% vs. 99.25% ）and in the high-quality embryo rate between group 3 and group 2 （ 37.14% vs. 44.42% ）（ P〈 0.05 ）. There were no significant differences in the rates of pregnancy and implantation among the three groups （ P 〉 0.05 ）. Conclusions Short-time insemination is beneficial for elevating the rates of the cleavage and high-quality embryo. The fertility rate of sperm and egg co-incubation for 4 h to 5 h is higher than that of co-incubation for 2 h to 3 h.