白细胞介素1受体拮抗剂基因多态性与复发性自然流产的关联性研究

Relationship between Polymorphism of Interleukin-1 Receptor Antagonist Gene And Recurrent Spontaneous Abortion

目的探讨白细胞介素1受体拮抗剂(Interleukin-1 Receptor Antagonist,IL-1Ra)基因多态性与宁夏汉族复发性自然流产(Recurrent Spontaneous Abortion,RSA)的相关性。方法应用聚合酶链反应(PolymeraseChain Reaction,PCR)技术检测191例反复自然流产患者和193例健康对照IL-1RN第二内含子中可变串联重复序列(Variable Number of Tandem Repeats,VNTR)多态性。结果 RSA组和对照组IL-1RN*1/1、IL-1RN*1/2、IL-1RN*1/3、IL-1RN*1/4、IL-1RN*2/2基因型频率分别为75.39%、20.42%、0、1.05%、3.14%和83.94%、11.92%、1.04%、2.07%、1.04%;等位基因IL-1RN*1、IL-1RN*2的频率分别为86.13%、13.35%和91.45%、6.99%,两组间差异有统计学意义(P<0.05)。携带有IL-1RN*2等位基因的个体发生复发性自然流产的危险性显著高于携带IL-1RN*1的个体(OR=2.03,95%CI:1.242～3.308)。结论 IL-1RN*2可能与宁夏汉族复发性自然流产的遗传易感性有关。

Objective To investigate the relationship between polymorphism of tandem repeat （VNTR） poly- morphism in intron 2 of interleukin - 1 receptor antagonist （ IL - 1 Ra） gene and recurrent spontaneous abor- tion. Methods 191 recurrent spontaneous abortion patients and 193 normal subjects were recruited in the study. Their VNTR polymorphisms of IL - 1Ra gene were analyzed using polymerase chain reaction method. Results The frequencies of the five genotypes were as follows： IL - 1RN ＊ 1/1,75.39% ;IL - 1RN ＊ 1/2, 20.42% ; IL - 1RN ＊ 1/3,0 ; IL - 1RN ＊ 1/4,1.05% ; IL - 1RN ＊ 2/2,3.14% in recurrent spontaneous abortion patients and IL - 1RN ＊ 1/1,83.94% ; IL - 1RN＊ 1/2,11.92% ; IL - 1RN＊ 1/3,1.04% ; IL - 1RN＊ 1/4, 2.07% ;IL- 1RN＊ 2/2,1.04% in normal subjects, respectively. The frequency of IL - 1RN＊ 1 and IL - 1RN ＊2 in recurrent spontaneous abortion patients was significantly higher than that in normal controls. Con- clusion Allele IL - 1RN ＊ 2 of the interleukin - 1 receptor antagonist gene was related potentially with recur- rent spontaneous abortion.