摘要
目的:研究双歧杆菌对新生鼠坏死性小肠结肠炎模型中肠道组织Toll样受体(TLR)2、4、9表达的影响。方法:出生24 h内Sprague-Dewley新生鼠随机分为对照组、实验组、治疗组,每组12只。对照组由母鼠喂养,实验组采取人工喂养+缺氧冷刺激进行造模,治疗组采用人工喂养+缺氧冷刺激并添加喂养双歧杆菌处理(2次/日,1×108cfu/次)。每日定时称体重做记录,在实验第3天处死新生鼠。用HE染色病理切片观察新生鼠肠道病理变化,RT-PCR方法检测肠道黏膜TLR2、TLR4、TLR9的mRNA表达量。结果:3组小鼠体重比较均具有统计学意义,对照组体重增长高于实验组及治疗组,治疗组体重增长高于实验组(P<0.05);实验组病理评分高于对照组及治疗组,治疗组高于对照组(P<0.05);TLR4 mRNA相对表达量实验组高于对照组及治疗组(P<0.05),治疗组的TLR2 mRNA、TLR9 mRNA相对表达量均高于实验组,TLR4 mRNA相对表达量低于实验组,这两组的3种受体mRNA相对表达量比较均具有统计学意义(P<0.05)。结论:双歧杆菌可减轻新生鼠坏死性小肠结肠炎肠道组织病理变化,作用机制可能与双歧杆菌能降低肠道黏膜的TLR4表达及增加TLR2、TLR9表达有关。
Objective ： To study the effect of bifidobacterium on the expressions of Toll - like receptor （TLR） 2, 4, and 9 in intestinal mucosa of neonatal rats with necrotizing enterocolitis. Methods： Sprague - Dewley neonatal rats within 24 hours after birth were randomly divided into control group, experimental group, and treatment group, 12 rats in each group. The neonatal rats in control group were fed by their mothers, the rats in experimental group were fed with artificial feeding combined with hypoxia and cold stimulation, and the rats in treatment group were fed with artificial feeding combined with hypoxia, cold stimulation and bifidobacterium （twice a day, 1 - 10s cfu per time） . Body weight was measured regularly every day, then the neonatal rats were killed on the third day. The intestinal pathological chan- ges of neonatal rats were observed by pathological smears after HE staining, RT - PCR was used to detect the expression levels of TLR2, TLR4, and TLR9 mRNA in intestinal mucosa. Results： There was significant difference in body weight among the three groups, the body weight gain in control group was significantly higher than those in experimental group and treatment group, and the body weight gain in treatment group was significantly higher than that in experimental group （ P 〈 0. 05 ） . The pathological score in experimental group was signifi- cantly higher than those in control group and treatment group, and the pathological score in treatment group was significantly higher than that in control group （ P 〈 0. 05 ） . The relative expression level of TLR4 mRNA in experimental group was significantly higher than those in con- trol group and treatment group （ P 〈 0. 05 ）, the relative expression levels of TLR2 mRNA and TLR9 mRNA in treatment group were signifi- cantly higher than those in experimental group, the relative expression level of TLR4 mRNA in treatment group was significantly lower than that in experimental group, there was significant difference among the three groups （ P 〈 0. 05 ） . Conclusion： Bifidobacterium can relieve the pathological change of intestinal tissue of the neonatal rats with necrotizing enterocolitis, the mechanism may be related to that Bifidobac- terium can reduce the expression of TLR4 mRNA and increase the expressions of TLR2 and TLR9 in intestinal mucosa.
出处
《中国妇幼保健》
CAS
北大核心
2012年第16期2505-2509,共5页
Maternal and Child Health Care of China
基金
广东省社会发展领域科技计划项目〔83062〕
