海带多糖的分类提取、鉴定及理化特性研究

Studies on Extraction, Identification, Physical and Chemical Characteris tics Analysis of Polysaccharides in Laminaria japonica Aresch

建立了一套简便有效的提取方案，可从海带（Ｌａｍｉｎａｒｉａ ｊａｐｏｎｉｃａ Ａｒｅｓｃｈ）中分类提取同时得到褐藻酸钠、褐藻糖胶、褐藻淀粉三类多糖粗品。将含量较高的前二者进一步纯化，乙酸纤维膜电泳和葡聚糖凝胶Ｇ－７５柱层析的结果表明纯化后的褐藻酸钠、褐藻糖胶是均一的。将二者与市售海藻酸钠试剂的光谱行为和理化特性进行了对比实验，结果表明市售海藻酸钠试剂与褐藻酸钠的紫外和红外图谱特征一致，分子量、特性粘度、电导率及ｐＨ值等数值均很接近；而与褐藻糖胶的性质有较大差异。

In this paper, a high efficient procedure was studied to extract crude sodium alginate, fucoidin and laminarin simultaneously. Sodium alginate and fucoidin were further purified, the purity of which was demonstrated homogeneously by chromatography on a sephadex G-75 column and acetate cellulose membrane electrophoresis .The experiments compared among soding alginate market Sodium alginate specifically prepared in the experiment and fucoidin.The results of the first two showed that their features on IR and UV spectrum were consistent and their datum on molecular weight, inherent viscocity electrical conductivity and pH were very close. However both differed greatly with fucoidin.