植物乳杆菌的降胆固研究

Cholesterol-Reducing of Lactobacillus Plantarum

对植物乳杆菌MA2的体外和体内降胆固醇活性进行了研究,结果显示:培养基中添加的胆固醇的初始浓度对植物乳杆菌MA2的胆固醇去除率影响显著,当胆固醇的终质量浓度为30mg/dL,不添加胆盐的条件下,菌株MA2的胆固醇去除率达到(55±4.5)%。死菌体和休眠菌体也可去除培养基中的胆固醇,且菌体细胞浓度的影响显著。添加胆盐对菌株MA2的胆固醇去除率影响显著。体外实验表明植物乳杆菌MA2通过吸附胆固醇,进而将胆固醇吸入菌体细胞中来降低培养基中的胆固醇。MA2菌株可显著降低大鼠血清总胆固醇、甘油三酯的水平,表明其作为降血脂的益生菌的应用潜力。

Lactobacillus plantarum MA2＇s vitro and vivo cholesterol-lowering activity were studied in this experiment. The results depicted that the percentage of cholesterol removed from MRS-CHOL broth might reached up to （55±4.5）% at 24^th h, when cholesterol concentration was 30 mg/dL and no bile salt was added. The resting and heat-killed cells showed the ability to remove cholesterol, and the influence of the biomass concentration was significant. Also, the effect of bile salts on cholesterol removal was also investigated. These findings suggested that Lactobacillus plantarum MA2 could remove cholesterol mainly via binding, absorbing cholesterol into cell and by assimilation. Moreover, the results showed that serum cholesterol and triglycerides were reduced significantly （P〈0.05） in comparison with the control, and indicate the probiotic potential of the Lactobacillus plantarum MA2 strain in hypocholesterolemic effect.