金丝小枣类黄酮提取最佳条件及抗氧化研究

Studies on the Extracting and Antioxidant Activities of Flavonoids in 'Jinsixiaozao'

为研究从金丝小枣中提取类黄酮的最佳方法及该提取物的抗氧化活性,采用四因素三水平正交试验方案,研究水浴法和超声法提取类黄酮的最佳条件,并对这两种条件进行比较。通过测定类黄酮清除1,1-二苯代苦味酰基自由基(DPPH)能力和铁还原能力(FRAP),分析金丝小枣提取物中类黄酮的抗氧化活性。试验结果表明:水浴法:在料液比为1:50的条件下,用60%乙醇70℃提取2.5h,提取2次,类黄酮含量为10.25mg/g;超声法:在料液比为1:40的条件下,用60%乙醇,超声功率400W,提取40min,提取2次,类黄酮含量为11.08mg/g。抗氧化试验表明:金丝小枣类黄酮具有一定的抗氧化能力,其抗氧化活性随浓度的升高而增强。

Extraction of flavonoid from ＇Jinsixiaozao＇ and the antioxidant activities of the extracts were studied in this paper. The withdrawing conditions of flavonoid by using water bath and ultrasonication were campared. The antioxidant properties including the capacities of scavenging DPPH and the ferric reducing activity power were investigated. The result shows that the best condition of water bath for withdrawing with 50 times 60% alcohol to ＇Jinsixiaozao＇, 70 ℃, withdrawing for 2.5 h, the extraction repeated for two times. The total flavonoids in the extract was 10.25 mg/g under the best condition. And the best condition of ultrasonication with 40 times 60% alcohol to ＇Jinsixiaozao＇, 400W, ultrasonic- ing 40 min, the extraction repeated for two times. The total flavonoids in the extract was 11.08 mg/g under the best con- dition. The anti-oxidizes the experiment enunciation： The flavonoid in ＇Jinsixiaozao＇ has certain antioxidant activities. The antioxidant activity improves with the rising of consistency.