Nuclisens EasyQ HIV-1和COBAS AMPLICOR HIV-1 MONITOR^(TM) Test检测集合在HIV-1“窗口期”诊断的比较应用

Comparative application of Nuclisens EasyQ HIV-1 and COBAS AMPLICOR HIV-1 MONITORTM Test in pooled test for detection of HIV-1 window period

目的将Nuclisens EasyQ HIV-1方法与COBAS AMPLICOR HIV-1MONITORTM Test方法检测集合的结果进行比较,并将集合HIV RNA Nuclisens EasyQ方法应用于艾滋病病毒Ⅰ型(HIV-1)"窗口期"的检测。方法采用50:1、10:1二级集合HIV RNA COBAS和EasyQ方法进行检测。结果 (1)两种方法在54份定值血清和质控血浆的阳性检出率均为100%,方法的一致性为94.44%,两方法之间具有显著相关性,相关系数为r=0.855。(2)检测感染者配偶、静脉吸毒者(IDU)及男男性行为人群(MSM)样本集合2 426人份,两种方法检测结果一致,发现3例"窗口期"感染者。结论集合HIV RNA Nuclisens EasyQ方法具有很好的实验性能,可应用于HIV高危人群的"窗口期"检测。

Objective To compare the results of Nuclisens EasyQ HIV-1 and COBAS AMPLICOR HIV-1 MON ITORTM Test in pooled test and to explore the application of HIV RNA Nuclisens EasyQ method in detecting HIV 1 window period. Methods Two levels （50：1, 10：1） of pooled method were used. Results （1） 54 valid value sera and quality control plasma were tested, the positive detection rates of the two methods were 100%, the consistency rates of them were 94.44%, and the correlation was significant with a correlation coefficient of R= 0 . 855. （2） 2426 samples from spouses of HIV infectors, IDUs and MSM were tested; the two methods had consistent results and 3 HIV infected individuals were detected during the window period. Conclusion HIV RNA Nuclisens EasyQ pooled test had good performance and could be applied in detecting window period in HIV high-risk population groups.