外源性环核苷酸调控哈白兔血脂、血糖及血蛋白的动态变化研究

Exogenous CNT Regulation Research on Dynamic Variation of Blood Fat, Sugar and Protein in Harbin white Rabbits

为了研究外源性环核苷酸对动物体内血脂、血糖及血蛋白的动态变化规律的调控作用,试验以哈白兔为试验动物,通过外源注射环鸟苷酸、环腺苷酸及其等量混合物的腹腔注射液,研究了不同处理组4天内不同时间点动物血脂、血糖及血蛋白的动态变化规律。结果发现：cGMP单独处理后,血脂含量大幅度增加,平均增加了17.63%,而经过cAMP处理后的血脂含量有较小幅度的减小;cAMP单独处理后,血糖含量平均增加了1.79%,而cGMP单独处理后血糖含量明显增加,增加率超过了10%;经过cGMP、cAMP和混合处理后血蛋白平均增加率大约为5%左右。此外,cGMP对促进脂肪的合成代谢有明显作用,通过注射cGMP在10 h内对血脂没有影响,10~21 h对血脂的合成有显著促进作用,21 h后血脂才保持一定的增加幅度;而cAMP对降解有较小作用,通过注射cAMP在45 h以内对血脂有降解作用,而45~65 h又转为合成作用,65 h以后作用消失;注射CNT 6~21 h时,糖异生作用促进了动物体内血糖含量的增加;注射CNT对血蛋白的作用不明显。这些主要代谢物质的动态变化规律为应用CNT调控动物体内营养物质代谢水平提供了有利的理论依据。

The aim of our study was to find out the regulation law of exogenous CNT（including cyclic Guanosine Monophosphate and cyclic Adenosine Monophosphate） on blood fat,sugar and protein in Harbin white rabbits.We studied the dynamic variation law of different treatment groups in different time points within 4 days through injecting cGMP,cAMP and cGMP ＋ cAMP.The results were as follows： blood fat content increased rapidly after injecting cGMP alone,about 17.63% in average,whereas after injecting cAMP the blood fat content decreased slightly.Increase of blood sugar content was only 1.79% in average after injecting cAMP alone,whereas after injecting cGMP the blood sugar content increased more than 10% in average.Increase of blood protein content was about 5% in average after injecting cGMP,cAMP and cGMP＋cAMP.In addition,cGMP has a key role in accelerating fatty synthesis metabolism,blood fat content did not change during the first 10 h of injecting cGMP,but significant effect on accelerating fatty synthesis was observed between 10 and 21 h,and blood fat kept rising in certain rate after 21 h of injection.But cAMP had a little effect in fat degradation.It had a fat degradation role with 45 h of injecting cAMP,but changed into fat synthesis role between 45 and 65 h,and the effect disappeared later.Furthermore,the effect CNT has little effect on blood protein,but gluconeogenesis resulted in the increase of blood sugar between 6 and 21 h of injecting CNT.Finally,the dynamic variation law of these main metabolites provide beneficial theoretical basis in CNT regulation and control of the nutritive metabolic level in animals.