新型八核铜配合物乳剂的体内外抗肿瘤作用

Study on anti-tumor effect of a new eight-copper complex emulsion in vitro and in vivo

目的探讨新型八核铜配合物乳剂(N-Cu)的体内、外抗肿瘤作用。方法采用甲基噻唑基四唑(MTT)法检测不同浓度N-Cu对HT29、HeLa、SGC7901和EC9706肿瘤细胞增殖的影响。利用S180腹水瘤小鼠模型观察不同浓度N-Cu的体内抗肿瘤作用。采用流式细胞仪测定不同浓度N-Cu对S180细胞周期的影响。结果 N-Cu作用4种肿瘤细胞24h后,细胞增殖均受到了不同程度的抑制,且呈浓度依赖性。N-Cu对荷S180小鼠的肿瘤生长有明显的抑制作用。N-Cu能使G0/G1期细胞明显增多,S期细胞减少,细胞被阻滞于G0/G1期,差异具有统计学意义(P<0.05)。结论 N-Cu在体内、外具有抗肿瘤作用。

Objective To study the anti-tumor effect of a new eight-copper complex emulsion（N-Cu） in vitro and in vivo. Meth- ods The effect of N-Cu on proliferation of HT29,Hela,SGC7901 andEC9706 cancer cell lines were evaluated by MTT reduction assay. The in vivo anti-tumor effect was examined in tumor-bearing mice. Cell cycle was determined by flow cytometry. Results N- Cu significantly inhibited the proliferation of the four tumor cell lines after 24 h. With increasing drug concentration, the inhibition was significantly enhanced. The results in vivo showed that N-Cu had better anti-tumor effects. N-Cu by flow cytometry showed that N-Cu with anti-S180 cell proliferation,an increase in Go/GI phase and decrease in S phase. Cells were arrested in Go/G1 phase. The difference was statistically significant（P〈0.05）. Conclusion N-Cu possesses the anti-tumor effect in vivo and in vitro.