鼻咽癌组织中微淋巴管和微血管密度的观察及其临床意义

Observation and significance of lymph vessel density and micro-vessel density in nasopharyngeal carcinoma

目的观察鼻咽癌组织中以D2-40标记的微淋巴管和以CD34标记的微血管的密度及其临床意义。方法对106例初治鼻咽癌的组织标本,用免疫组化的方法及计算机辅助的图像分析系统定量分析肿瘤组织中淋巴管密度(LVD)及微血管密度(MVD)与临床资料的关系,同时分析LVD与MVD之间的关系。结果 106例标本淋巴管和微血管染色均呈阳性。LVD与N分期、复发及转移相关(P<0.05),而与年龄、性别、T分期、临床分期无关(P>0.05)。MVD与T分期、临床分期、复发及转移相关(P<0.05),与年龄、性别、N分期无关(P>0.05)。CD34的表达与D2-40的表达呈正相关。结论 LVD是鼻咽癌颈淋巴结转移的重要因素,LVD、MVD均与鼻咽癌复发及远处转移密切相关。

Objective To assess the expression and significance of lymph vessel（LVD） density marker with D2-40 and micro- vessel density（MVD） marked with D34 in nasopharyngeal carcinoma（NPC）. Methods 106 NPC patiens pathologically proved and treated in our hospital were included. Immunostaining for the lymphatic marker D2-40 and for the vascularendothelial marker CD34 were used in same NPC specinen. Computer-assisted morphometric analysis was used. The lymph vessels and blood vessels were quantified within the tumor area in all patients in the same specinen. Results Both lymph vessels and blood vessels within the tumor were found. A significant correlation was found between the expression of lymph vessels and N stage, recurrence and metas- tasis（P〈0.05）. No significant correlation was found between the lymph vessel parameters and age,gender, T stage and clinical stage（P〉0.05）. There was a significant correlation between the blood vessel parameters and T stage, clinical stage, recurrence and metastasis（P〈0.05）. No significant correlation was found between the blood vessel parameters and age,gender, N stage（P〉0. 05）. The expression of D2-40 had positive correlation with the expression of D34. Conclusion LVD is the factors of neck lymph node metastasis. Both LVD and MVD are related with recurrence and metastasis of NPC.