降浊升清汤对Apo-E基因敲除小鼠主动脉粥样硬化炎症反应的影响

Effect of Jiangzhuo Qingyang Decoction on Inflammatory Response of Apo-E Knockout Mice Aortic Athrosclerosis

目的:探讨降浊升清汤对Apo-E基因敲除小鼠主动脉粥样硬化(As)炎症反应的影响及其稳定As斑块的作用机制。方法:将35只6～8周龄Apo-E基因敲除小鼠予高脂饮食喂养6周后,随机处死5只,取主动脉根部,HE染色普通光镜下观察,确定As形成后,将剩余30只小鼠随机分为3组:实验组(降浊升清汤组)、中药对照组(复方鳖甲软肝片组)、西药对照组(辛伐他汀组)各10只,3组小鼠分别给药6周后处死,检测血清中IL-6、TNF-α的变化情况。结果:与中药对照组比较,实验组、西药对照组对Apo-E基因敲除小鼠血清IL-6、TNF-α的含量明显降低。结论:降浊升清汤具有抗动脉粥样硬化的作用,其机制可能与下调IL-6、TNF-α的表达有关。

Objective： To explore the effect of Jiagzhuo Qingyang Decoction on inflammatory response and its stable AS plaque mechanism of Apo-E knockout mice aortic athrosclerosis.Methods： 35 Apo-E knockout mice of 6-8 weeks were fed with high fat diet for 6 weeks.The aortic roots of the 5 killed mice were observed with common HE-dyed microscope.The rest 30 mice were randomly divided into three average groups（the treatment group with Jiagzhuo Qingyang Decoction,TCM control group with Compound Biejia Rungan Tablets,Western medicine group with DMsin） and they were killed 6 weeks later.The changes of IL-6 and TNF-αin the serum were detected.Results： The contents of IL-6 and TNF-αin the serum of Apo-E knockout mice in the treatment group and Western medicine control group have obviously reduced than that of TCM control group.Conclusion： Jiagzhuo Qingyang Decoction has anti-athrosclerosis effect and its mechanism may be relevant to the expression of low regulating IL-6 and TNF-α.