深圳地区无偿献血者群体CD36抗原缺失型的表型分析

The phenotype of platelet CD36 deficiency in the blood donors of Shenzhen,China

目的分析深圳地区无偿献血者群体CD36抗原缺失型的筛查和表型情况。方法应用单克隆抗体和流式细胞技术在327名献血者中检测CD36抗原缺失型个体,应用血小板单克隆抗体捕获酶联免疫技术和流式细胞术筛查献血者血浆中的血小板抗体。结果此次筛查得到的深圳献血者的CD36抗原缺失型频率为3.06%(10/327),其中Ⅰ型占0.31%(1/327),Ⅱ型占2.75%(9/327);在3名女性CD36抗原缺失型献血者的血浆中,均未检出血小板反应性抗体,包括抗-CD36。结论深圳地区献血者群体中存在CD36抗原缺失型个体,其频率与亚洲其他地区人群的频率相当;对CD36的同簇免疫应该予以重视。

Objective To analyze the phenotype and frequency of the CD36 deficiency among the volunteer blood donors in Shenzhen. Methods The phenotypes of CD36 deficiency were analyzed by monoclonal antibodies and flow cytome- try （FCM）. Platelet-reactive antibodies in the plasmas of suspected donors were screened by the antigen capture enzyme- linked immmnosorbent assay and platelet suspension immunofluorescenee test. Results The frequency of CD36 deficiency among blood donors was found to be 3.06% （ 10/327 ）, and the frequencies of type I and II deficiencies were found in 0. 31% （ 1/327 ） and 2.75% （9/327）, respectively. No platelet-reactive antibodies were detected in the plasmas of 3 female c donors with CD36 deficiency. Conclusion The frequencies of CD36 deficiency in blood donors of Shenzhen are found that the same ranges with the other Asians. These results suggest the necessity to concern the clinical effects of isoimmunization caused by CD36 deficiency in transfusion medicine.