观赏金银花中绿原酸的超声提取研究

A Study of Extracting Chlorogenic Acid from Spectacular Honeysuckle by Ultrasonic

以观赏金银花为原料,利用超声波强化提取绿原酸化合物.在单因素试验的基础上,采用正交试验法,考察了超声时间、料液比、乙醇浓度、超声温度对绿原酸提取率的影响,确定最佳的提取工艺.结果表明:超声波提取观赏金银花中绿原酸的最佳工艺条件为乙醇浓度60%,料液比1∶20,超声温度70℃,超声时间40 min.在上述最佳条件下,绿原酸的提取量为36.72 mg/g.

The extraction of chlorogenic acid from spectacular honeysuckle with ultrasonic wave was studied.On the basis of mono factor and orthogonal experimental design,the influence of several parameters on the extraction rate of chlorogenic acid was investigated,including ultrasonic time,material/extraction solution ratio,ethanol concentration,extraction temperature.The optimal extraction process was determined.The result shows that the optimum extraction conditions extracting of chlorogenic acid from spectacular honeysuckle by ultrasound were as follows：ethanol concentration 60%,extraction temperature 70 ℃,ultrasonic time 40 min and ratio of liquor to material 20 mL/g.Under the above optimum conditions,the chlorogenic acid extracted from spectacular honeysuckle was yielded by 36.72 mg/g.