一种适用于高分辨分子地层学研究的有机质分离及定量方法

A Quantitative Method Applicable to High Resolution Molecular Stratigraphy

研究全球变化的手段多种多样，高分辨分子地层学是最有力的工具之一，进行此项研究工作要分析的样品数量大且容量小，因此建立一种合适的有机质分离及定量方法十分重要。本文采用冷抽提的方法抽提出这类样品的有机质，衍生化后利用GC／MS对化合物进行定性、定量分析。利用本方法分析的空白加标（地质体中最常见化合物）回收率结果为：烷烃95．8％，脂肪酸92，3％，胆甾醇99．7％，脂肪醇93．7％；基质加标回收率结果为：烷烃94．8％，脂肪酸90．8％，胆甾醇98．8％。由此可见，此方法可用于沉积物中常见化合物定性定量分析。

The research of high resolution molecular stratigraphy is able to provide plenty of information about global climatic change. To do this research, we have to analyze a great number of samples in quite small amount. The present paper describes a quantitative method for this analysis. The organic lipids tere extracted from the sediments by the suspension. The resultig lipids were derivatized with bis-trimethylsilyltrifluoroacetamide and subject to the analysis of GC/ MS - With adding standards to the blank, the recovery of n-alkane, fatty acid, cholesterol and alcohol is 95. 8%, 92. 3%, 99. 7% and 93. 7% respectively. With adding standards to the mineral matrix, the recovery of n-alkane, fatty acid, cholesterol is 94 . 8%, 90. 8% and 98. 8% respectively. These asults show this procedure is applicable to the qualitative and quantitative analysis of these compounds in the sediments.