用SEC柱和胶束流动相分离小分子的模型

Separation Model of Small Molecules with SEC Columns and Micellar Mobile Phase

使用体积排阻色谱 (SEC)柱和胶束流动相成功地分离了一些小分子化合物 ,给出了分离模型。这种分离方式是基于胶束和水相在色谱过程中的不同迁移以及溶质在胶束和水相间的不同分配而实现的 ,其分离的机制与胶束电动色谱 (micellar electrokinetic chromatography,MEKC)十分相似。理论处理的结果表明 ,溶质的保留体积与胶束浓度有关 ;通过溶质的保留体积可得到溶质在胶束和水相间的分配系数。还采用了两种不同的 SEC柱分离了一些脂肪醇 ,验证了这一理论模型 ,测定了它们的分配系数 ,结果表明两种柱测得的小分子醇在胶束和水相中的分配系数具有较好的一致性。

Small alkanol molecules were successfully separated with SEC method in combination with a micellar mobile phase.The separation model has been given in this system.The separation is based on the differential migration of the micelle and the surrounding water and the differential distribution of the solute between the micelle and aqueous phase,therefore,the separation mechanism is very similar to that of micella electrokinetic chromatography(MEKC). The retention volume of solute is related to the micellar concentration by theoretical treatment,through which the solute distribution coefficient between micelle and aqueous phase could be obtained.Two different SEC columns were employed for separation of some alkanols and the theoretical model is supported by the experimental results. The distribution coefficients obtained were in good agreement for these two columns.