新型手性配体交换色谱固定相的制备及应用

Preparation of Novel Chiral Stationary Phases for Ligand Exchange Chromatography and Their Applications

合成了 2 -(2 -羟基 -3 -烷氧基 )丙基 -(S) -1 ,2 ,3 ,4-四氢 -3 -异喹啉羧酸手性选择子 ,制备了两种新型涂渍手性配体交换色谱固定相 ,拆分了某些 DL-氨基酸 ,比较了 DL-氨基酸在两种手性相上的色谱分辨。

Two novel chiral stationary phases for ligand exchange chromatography were prepared by synthesizing 2-(2-hydroxyl-3-octoxy)propyl-(S)-1,2,3,4-tetrahydro-3-isoquinolinecarboxylic acid (C_8-(S)-THIQCA) and 2-(2-hydroxyl-3-dodecoxy)propyl-(S)-1,2,3,4-tetrahydro-3-isoquinolinecarboxylic acid (C_12-(S)-THIQCA) as chiral selectors, and coating them on ODS bonded stationary phase. Chromatographic resolutions of some DL-amino acids were achieved on the chiral stationary phases by using 1 mmol/L NH_4Cl with 0.2 mmol/L Cu(Ac)_2solution as mobile phase and detection at 254 nm. Ability of chiral resolution of the DL-amino acids through the stationary phases was compared and the effect of cupric ion on resolutions of the DL-amino acids was examined. The elution order of D-isomer before L-isomer was observed for all the DL-amino acids resolved, except DL-Val For DL-Pro, DL-Val and DL-Leu the elution order through the chiral phases was different from that through the chiral ligand exchange phases prepared from L-Pro or L-hydroxyl proline with five membered ring structure.