毛细管电动色谱带电环糊精拆分N-FMOC氨基酸对映体

Enantiomeric Separation of N-FMOC Amino Acids with Sulfobutyl Ether-β-Cyclodextrin as Chiral Additive by Capillary Electrokinetic Chromatography

将负电性磺丁基 -β -环糊精手性添加剂应用于毛细管电泳氨基酸对映体的拆分研究中 ,对 8种氨基酸对映体与 9-芴甲基氧基甲酰氯 (FMOC-Cl)生成的衍生物进行了分离 ,其中 5种得到了基线分离。考察了背景电解质 p H值及磺丁基 -β -环糊精的浓度对 N-FMOC氨基酸对映体拆分的影响。

Enantiomeric separation of N\|FMOC amino acids by capillary electrophoresis with an anionic chiral resolving agent, sulfobutyl ether-β-cyclodextrin(SBE-β-CD) with average substitution degree of 3.8, employed as chiral additive was studied. Chiral separation was achieved with the mode of electrokinetic chromatography due to the electrophoretic mobility and chiral complexing ability of the chiral additive. The effects of pH of background electrolyte, the concentration of sulfobutyl ether-β-cyclodextrin on enantiomeric separations were investigated. The enantiomeric separations of 8 N\|FMOC amino acids were accomplished, among which 5 N\|FMOC amino acids could be on baseline separated.