高效液相色谱法测定人血浆中的芦氟沙星

Determination of Rufloxacin in Human Plasma by High Performance Liquid Chromatography

建立了测定人血浆中芦氟沙星质量浓度的高效液相色谱法 ,血浆用二氯甲烷提取 3次 ,以 UltrasphereODS(4.6mm i.d.× 2 5 0 mm)为色谱柱 ,流动相为甲醇 -1 0 mmol/L 溴化四丁铵 -三乙胺 (体积比为 3 2∶ 68∶0 .5 ) ,用磷酸调 p H2 .8,检测波长 2 95 nm,流速为 1 .2 m L/min,以培氟沙星为内标。血浆中芦氟沙星的线性范围为 0 .1～ 1 0 mg/L ,最低检测质量浓度为 0 .0 5 mg/L ,回收率为 99.7% ,日内、日间 RSD分别为 2 .3 3 %和3 .83 %。方法简便、快速、准确 ,适用于人血浆中芦氟沙星质量浓度的测定。

A high performance liquid chromatographic method has been developed for the determination of rufloxacin in human plasma. Rufloxacin was extracted from plasma with dichloromethane for three times. It was chromatographed on an Ultrasphere ODS column with Pefloxacin as internal standard with a mobile phase consisting of methanol\|tetrabutylammonium bromide\|triethanolamine (32∶68∶0.5,V/V) adjusted to pH 2.8 with orthophosphoric acid. The flow rate was 1.2 mL/min and the monitoring wavelength was 295 nm. The calibration curve was linear from 0.1 to 10 mg/L of plasma. The detection limit of rufloxacin was 0.05 mg/L for plasma and the recovery was (97.7±2.1)%. The intra\|day RSD and inter\|day RSD were 2.33% and 3.38% respectively. The method is simple, rapid, accurate and can be used to determine the rufloxacin concentration in plasma and for pharmacokinetic study.