布渣叶的化学成分研究

Study on chemical constituents of Microctis Folium

目的研究布渣叶Microcos paniculata的化学成分。方法采用硅胶、Sephadex LH-20、大孔树脂等柱色谱及制备液相色谱等方法进行分离纯化,根据化合物的理化性质、波谱数据等鉴定结构。结果从布渣叶的醋酸乙酯和正丁醇萃取部位分离并鉴定了12个化合物,分别为异鼠李素(1)、表儿茶素(2)、黑麦草内酯(3)、去氢吐叶醇(4)、香草酸(5)、丁香酸(6)、咖啡酸甲酯(7)、对羟基苯甲酸(8)、对香豆酸(9)、木栓醇(10)、豆甾醇(11)、β-谷甾醇(12)。结论化合物3～7、9～12均为首次从该属植物中分离得到,其中化合物3和4为首次从该属植物中分离得到的单萜类化合物。

Objective To study the chemical constituents of Microctis Folium（the leaves of Microcos paniculata）.Methods The chemical constituents were isolated and purified by column chromatography on silica gel,Sephadex LH-20,macroporous resin,and preparative HPLC,etc.The structures of compounds were elucidated by spectral analysis,comparing physicochemical properties,and TLC with authentic samples.Results Twelve compounds were isolated from the EtOAc and n-butanol fractions of Microctis Folium,and identified as isorhamnetin（1）,epicatechin（2）,loliolide（3）,（＋）-dehydrovomifoliol（4）,vanillic acid（5）,syringic acid（6）,methyl caffeate（7）,p-hydroxybenzoic acid（8）,p-coumaric acid（9）,friedelinol（10）,stigmasterol（11）,and β-sitosterol（12）.Conclusion Compounds 3—7 and 9—12 are isolated from the plants in Microctis L.for the first time.Compounds 3 and 4 are firstly reported monoterpenes in this genus.