白菜重要园艺性状的QTL定位分析

QTL Analysis of Important Horticultural Traits of Chinese Cabbage-pak-choi

为从转录水平上准确定位显著影响各性状的QTLs,及其遗传效应和互作规律,以矮脚黄白菜自交系(97-3-2)和白蔓菁芜菁自交系(001-24)为亲本及其杂交建立F6重组自交系群体为材料,采用cDNA-AFLP、cDNA-SRAP技术,构建了一个总长度为1 048.4 cM包含311个分子标记,14个连锁群的高密度转录图谱。并采用基于混合线性模型的QTL作图分析软件QTLmaper2.0对F6群体进行QTL作图分析,检测到控制蛋白质、Vc、株高、伸展度、叶片数、单株质量等12个主要园艺性状的42个QTL,定位于分子转录图谱的14个连锁群中,并阐明了单个QTL的遗传效应及QTLs间的互作遗传效应。

In order to exactly locate QTLs significantly affectting various characters,their hereditary effects and interacting rules at the transcription level,in this study,the inbred line ＇Aijiaohuang 97-3-2＇of Chinese cabbage-pak-choi Brassica campestris L.ssp.chinensis Makino,the inbred line ＇Baimanjing 001-24＇ of turnip B.campestris ssp.rapifera（Matzg.） Sinsk and F6 recombinant inbred（RIL） population were used as the experimental materials,adopting cDNA-AFLP and cDNA-SRAP technique,a high-desity transcription map with 1 048.4 cM length,comprised of 311 molecular markers and 14 linkage groups was constructed.And using mapping analysis software of QTLmapper2.0 based on mixed linear model the QTL mapping analysis of the F6 group was conducted,42 QTLs controlling 12 horticultural traits including protein,Vc,plant height,stretch degrees,etc were detected,and located on the 14 linkage groups,the hereditary effect of a single QTL and interactions genetic effects of QTLs were figured out.