凤仙透骨草提取液抑菌特性的研究

Studies on the Antimicrobial Characteristics of Extracts from Impatiens balsamina L.

以凤仙透骨草φ=95%乙醇提取液为研究对象,研究其对6种真菌、2种革兰氏阳性菌和2种革兰氏阴性菌的抑制效果,并研究不同因素对其抑菌效果的影响。结果表明,凤仙透骨草提取液具有广谱抗菌性,对供试的霉菌、酵母、革兰氏阴性和阳性菌均表现出了抑菌活性,其中对真菌的抑制效果优于细菌,对大肠杆菌的抑制活性较弱;提取物具有良好的热稳定性和耐贮藏性,提取物4℃贮藏100 d或经121℃高温湿热灭菌1 h后,抑菌活性变化很小;紫外光对抑菌物质有一定影响,应适当避光保存;提取液在酸性、弱酸性及中性环境条件下抑菌活性高,当pH值大于12时,抑菌活性显著下降;二价阳离子对抑菌效果有一定影响,三价Fe3+影响最大,应避免用金属容器保存提取液。

Antimicrobial characteristics of 95% ethanol extracts from the stems of Impatiens balsamina L.（EIB）,a traditional Chinese herbal medicine,were studied in order to provide a basis for the development of botanical fungicides and preservatives.The antimicrobial activities of the extracts against 4 moulds,2 yeasts,2 Gram-positive bacteria and 2 Gram-negative bacteria,as well as the factors which effect the inhibitory stability,were observed.The EIB was observed to show effective antimicrobial activities and broad antimicrobial spectrum against all tested fungi and bacteria species.The extracts showed good thermal stability and storability property for the antimicrobial activities decreased little after storage for 100 days or sterilized for 1 hour in an autoclave under 121 ℃.The UV exposure time and concentration of Fe3＋ affected the inhibitory activities significantly.Meanwhile,the extracts showed good activities under the pH range 2~8.In conclusion,the EIB may be selected as a natural preservative and antibacterial agent.