半枝莲多糖的纯化及抗肿瘤作用的体内实验研究

Experimental Study on Anti-tumor Effects of Scutellaria barbata D.Don Polyaccharides in Vivo

初步探究半枝莲多糖的纯化方法及其对动物体内的抑瘤作用。通过Sevag法纯化半枝莲多糖,并采用苯酚-硫酸法测定多糖含量;动物移植性肿瘤实验观察半枝莲多糖对小鼠体内肿瘤细胞生长和免疫器官—胸腺、脾的影响。多糖含量提高到49.99%;对S180肉瘤生长的抑制率以中剂量为佳;各剂量组均对脾脏指数有显著性差异,但大剂量注射(200 mg/kg),对胸腺有抑制作用。Sevag法处理后的纯多糖含量与粗多糖中的多糖含量相比有明显差异,该法切实可行;半枝莲多糖对S180小鼠有抗肿瘤作用和免疫增强作用。

Objective：To study the purifying method for Scutellaria barbata D.Don polyaccharides（SPS） and its anti-tumor effect of SPS in entrails.Methods： Using Sevag and Phenol-sulfuric acid method the content of polysaccharide was determined;the anti-tumor effect of SPS and the spleen index and the thymus index were observed by tumor transplant experiment on animal.Results：SPS can inhibite the growth of S180 and the effect on middle dose group was the best;the spleen index was affected remarkably in every group and the thymus index was inhibited by big dose（200 mg/kg）.Conclusion：The content of polysaccharide purified with Sevay method and the polysaccharide content in crude polysaccharide were significantly different;the method is practical;SPS has anti-tumor effect and can improve immunity.