HPLC法测定消肿止痛巴布膏中栀子苷的含量

Determination of Gardenoside in Xiaozhong zhitong cataplasm by HPLC

目的:建立HPLC法测定消肿止痛巴布膏中栀子苷的含量。方法:色谱柱采用Kromasil C18柱(250mm×4.6mm,5μm);流动相:甲醇-水(27∶73),检测波长:240nm;流速:1.0ml·min-1;柱温:30℃;结果:栀子苷进样量在0.06552～1.3104μg范围内线性关系良好(r=0.9999,n=6);平均回收率为98.5%,RSD=1.61%(n=6)。结论:所建立的方法准确、简便、快速,适用于消肿止痛巴布膏质量控制。

Ojective： To establish an analytical method of determinating Gardenoside in Xiaozhong Zhitong cataplasm by HPLC. Methods： Gardenoside in the cataplasm were separated by HPLC on Cls column （250mm ~ 4. 6mm, 5μm） with a methanol -water （27：73） as mobile phase and detected at 240 nm. The flow rate was 1.0ml · min-1 and the column temperature was 30℃. Results： The calibration curves showed good linear over the range of 0. 06552 ~ 1. 3104txg, （r =0. 9999, n =6 ） for Gardenoside, the average recovery rate were 98.5% with RSD 1.61% （ n =6）. Conclusion： The method is simple, accurate, rapid and can be used to control the quality of the Xiaozhong Zhitong cataplasm.