HPLC法测定清热化毒丸中黄芩苷的含量

Determination of berberine hydrochloride in Jianpi Zengshi Wan by HPLC

目的:用HPLC法测定清热化毒丸中黄芩苷的含量。方法:采用高效液相色谱法,使用Agilent ZORBAX SB-C18(250mm×4.6mm,5μm)色谱柱,以甲醇-水-磷酸(47∶53∶0.2)为流动相,检测波长为315nm,流速:1.0mL·min-1,柱温:40℃。结果:黄芩苷在0.1028～1.0275μg范围内呈良好的线性关系,回归方程为y=10225920x-1083344(r2=0.9925)。平均回收率为99.61%,RSD=1.4%。结论:本法灵敏、准确、专属性强,回收率高,重现性好,可作为清热化毒丸中黄芩苷的含量测定的检测方法。

Objective： To establish the method for determining berberine hydrochloride in Qingre Huadu Wan by HPLC. Methods： Berberine hydrochloride was analysed simultaneously by HPLC. The optimal conditions of separation and detection were achieved on a Agilent ZORBAX SB - C18 （250 mm ×416 mm, 5μm ） by isocratic elution with the mobile phase of acetonitrile - water （47： 53 ） at the flow rate of 1.0 mL · min-1. The detective wavelength was 315nm. Results： The calibration curves of berberine hydrochloride showed that the linearity were completely acceptable. The average recovery of berberine was 99. 61% respectively. The RSD wasl. 4%. Conclusion： This method was simple, rapid and feasible for the quantification of berberine hydrochloride in Qingre Huadu Wall.