水稻突变体B1-24的细胞学观察及其激素响应

Study on the Cytology of Rice Mutant B1-24 and Its Responses to Phytohormones

以半矮化水稻突变体B1-24及其野生型惠阳珍珠早(Hyz)幼苗为材料,探究其细胞学特征及其对激素的响应.在光下生长的B1-24幼苗比Hyz矮14.62%,第一真叶长度短11.18%,黄化苗植株的中胚轴长度增长41.89%.B1-24黄化苗的胚芽鞘细胞长度仅为Hyz的45.95%,而中胚轴细胞长度是其118.28%.B1-24分别经激素GA3(600μg/mL)和IAA(7μg/mL)处理,其细胞长度显著增长,50μg/mL PAC处理使其严重缩短.推测B1-24的半矮化突变可能与激素合成或响应有关.

We investigated the cytological characteristics and hormone responses of semi - dwarf rice mutant B1 - 24 by comparing to the wild type Huiyangzhenzhuzao （Hyz）. Compared with Hyz, the height of B1 -24 seedlings grown in light was 14. 62% shorter and the length of the first leaves was 11.18% shorter; while the mesocotyl length of B1 -24 was 41.89% longer. In addition, the lengths of coleoptile and mesocotyl cells of etiolated B1 - 24 seedlings were 45.95% and 118.28% of that of Hyz, respectively. Treated with 600 μg/mL GA3 or 7 μg/mL IAA, the cell length of B1 - 24 was significantly increased to the same level of Hyz. While 50 μg/mL PAC could severely shorten all of them. Our results suggested that the semi - dwarf phenotypes of B1 - 24 may be related to the synthesis of, or response to phytohormone（s）.