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Bcl-2抑制剂S1诱导人卵巢癌细胞凋亡及其机制的研究 被引量：3

Research of mechanism that inhibitor of Bcl-2 S1 induce SKOV3 cell apoptosis
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摘要 目的:以人卵巢癌细胞SKOV3为研究对象,探讨Bcl-2抑制剂S1诱导人卵巢癌细胞凋亡的机制。方法:通过MTT法检测S1作用下卵巢癌细胞的生存率;S1 10μmol/L作用不同时间,流式细胞术检测细胞凋亡率;Western Blot检测凋亡相关蛋白Mcl-1的变化。结果:与对照组相比S1能明显抑制SKOV3的生存率;流式细胞术结果显示,S1引起SKOV3细胞凋亡明显升高;蛋白水平检测S1作用8 h能明显抑制SKOV3细胞Mcl-1蛋白表达。结论:S1可能通过抑制Mcl-1蛋白诱导人卵巢癌细胞凋亡。 Objective To investigate the apoptosis mechanism induced by micromolecule compound S1 in human ovarian cancer cells SKOV3. Method Cell viability was detected by MTT to S1 in ovarian cancer cells SKOV3 ; The apoptosis rate of SKOV3 cells was deter- mined by Flow Cytometry analysis; Western blotting was used to analyze the expression of the protein of Mcl - 1. Results MTr assay indicated that compared with control group,S1 can decrease the cell viability of SKOV3 cells. It significantly activated the apoptosis in SKOV3 ceils measured by Flow Cytometry analysis treated with S1. Western blotting showed the result that apoptosis - relative protein Mcl - 1 was inhibited by S1 in human Ovarian cancer cells. Condusion The main mechanisms that S1 induce SKOV3 cell apoptosis through the way that inhibit Mcl - 1 protein.
作者 琴瀚姣 刘宁 张晶 张钰 杨晓春 张志超 孙连坤
机构地区 吉林大学 吉林大学白求恩医学院病理生理学系 北华大学病理学系 大连理工大学精细化工国家重点实验室
出处 《吉林医学》 CAS 2012年第13期2693-2695,共3页 Jilin Medical Journal
基金 吉林大学"大学生创新性实验计划"项目(项目编号:2011A71126) 吉林省科技厅资助项目(项目编号:200705373)
关键词 人卵巢癌细胞 S1 MCL-1 凋亡 Ovarian cancer cells S1 Mcl - 1 Apoptosis
分类号 R737.31 [医药卫生—肿瘤]
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参考文献8

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共引文献2

同被引文献46

  • 1丁江华,张荣艳,彭燕.慢性粒细胞性白血病P-gp和Bcl-2表达及与病情进展的关系[J].江西医学院学报,2004,44(6):13-15. 被引量：1
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引证文献3

二级引证文献12

吉林医学
2012年 第13期

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