急性肺损伤新生大鼠肺表面活性蛋白A变化的研究

Changes of surfactant protein a after acute lung injury in neonatal rats

目的:探讨肺表面活性物质蛋白A(SP-A)在急性肺损伤(ALI)/ARDS发病机制中的作用及其在ALI早期诊断中的意义。方法:采用内毒素(LPS)腹腔注射的方法复制新生大鼠ALI模型,应用酶联免疫吸附法(ELISA)检测血清、BALF中的SP-A水平。结果:ALI组血清SP-A水平在1h已明显高于对照组(P<0.05),8h时达高峰值,之后虽略有下降,但仍明显高于对照组(P<0.05);ALI各组BALF中的SP-A水平与对照组相比,有下降趋势,但无统计学意义(P>0.05)。结论:ALI新生大鼠SP-A的变化,可导致PS各组分比例的改变,在ALI发病机制中发挥重要作用;ALI发病初期,血清SP-A水平即可出现显著升高,反映了肺泡上皮细胞的损伤和肺毛细血管通透性的增高。因此,检测血清SP-A水平可作为ALI早期诊断的指标。

Objective To explore the role of surfactant protein A （SP - A） in pathogenesis of acute lung injury （ALI） in neonatal rats. Method After endotoxin lipopolysaccharide （LPS） induced acute lung injury （ALI） in neonatal rats,levels of SP - A in serum and bromchoalveolar lavage fluid （BALF） were measured by using Enzymo -linked immtmoadsorbent （ELISA）. Results In ALI groups,level of SP - A in serum at 1 hour significantly increased than that of control group （P 〈 0.05）. The value reached peak at 8 hours. Then it decreased. Level of SP - A in BALF in all ALl groups did not significantly decrease than that of control group （ P 〉 0.05）. Conclusion - Changes of SP - A and PS are important for the pathogenesis of ALL In the early of ALI ,the level of SP - A in serum increases significantly. It reflects the injury of alveolar epithelial cells and the raising of the pulmonary capillary vessels＇ permeability. So the concentration of SP - A in serum can be an index on the early diagnosis of ALl.