烟熏联合脂多糖制备大鼠慢性阻塞性肺疾病动物模型

Establishment of rat chronic obstructive pulmonary disease model with cigarette inhalation and intratracheal instillation of LPS

目的通过动态观察大鼠慢性阻塞性肺疾病(COPD)动物模型制备过程中肺组织及小支气管的病理改变,以期进一步了解COPD疾病的发展过程。方法采用2次气道内注入细菌内毒素脂多糖和连续被动吸烟4周的方法建立COPD大鼠模型。造模过程中取第7、14、21、28、42天大鼠肺组织进行病理HE染色。结果造模28dCOPD大鼠模型均符合人类COPD的病理形态学特点,模型组大鼠在造模过程中逐渐出现肺气肿表现。结论气道内注入脂多糖与烟熏的复合方法能成功制备较稳定大鼠COPD动物模型。

Objective To dynamically observe the pathogenic changes of lung tissue and small bronchia during constructing process of the rat model of chronic obstructive pulmonary disease（COPD）to further understand the developing process of COPD.Methods The COPD rat model was established by cigarette smoking inhalation for 4 weeks and twice intratracheal instillation of lipopolysaccharide（LPS）.Then the model′s lung tissues were taken on 7,14,21,28,42 d for pathological HE staining respectively.Results The COPD rat model on 28 d accorded with the pathomorphological characteristics of human COPD.The rats of COPD models gradually developed the manifestations of emphysema during the model-constructing process.Conclusion The stable COPD rat model may be successfully established by this combined methods of cigarette smoking inhalation and intratracheal instillation of LPS.