高血压大鼠穹隆下器室管膜扫描电镜特征

Scanning electron microscopic observation on ependyma of subfornical organ in hypertensive rats

目的观察高血压大鼠穹隆下器室管膜游离面扫描电镜特征。方法选择雌性Wistar大鼠40只分为实验组(n=30)和对照组(n=10)。实验组予以左旋硝基精氨酸(L-NNA)15mg/(d.kg),分别于8:00、20:00腹腔注射,制备高血压动物模型。于用药2(10只)、4(10只)、8周(10只)常规经心灌注固定、取脑。对照组不做任何处理。用扫描电镜观察高血压大鼠穹隆下器室管膜的特征。结果扫描电镜下主要表现为室管膜细胞形态改变,过渡区室管膜上纤维网排列紊乱、断裂,甚至消失;中央区室管膜上分泌泡先增加后减少。结论高血压大鼠穹隆下器室管膜细胞和室管膜上结构存在超微结构的损伤,提示以上形态学的改变和高血压的变化有关。

Objective To observe the ependymal changes of subfornical organ（SFO）in the hypertensive rats by scanning electron microscope.Methods 40 healthy male Wistar rats were chosen randomly and divided into two groups：the experimental group（n=30）and the control group（n=10）.The experimental rats were treated with 15 mg/（d-1·kg-1）of L-nitroarginine（L-NNA）by twice intraperitoneal injection at 8：00 AM and 8：00 PM for preparing the animal model.The experimental group was performed the conventional cardiac perfusion and fixation in 2（10 cases）,4（10 cases）and 8 weeks（10 cases）after drug administration,then the rat brain was taken out.The control group had no treatment.The ependymal characteristics were observed by scanning electron microscope.Results The main manifestations of scanning electron microscope were the morphological changes of ependymal cells,which including disarranging,breakage or even disappearing of supraependymal fiber networks distributed in the rostral zone and transition zone of SFO,the secretory granules of the ependymal cells increased firstly and then decreased in quantity in the central zone.Conclusion The ultrastructure injuries of ependymal cells and supraependymal structures of SFO exist,which suggests that the changes might be related with hypertension.