上皮性钙黏蛋白与闭锁蛋白在慢性鼻-鼻窦炎上皮的表达及意义

The expression of E-cadherin and occludin in epithelium of chronic rhinosinositis and its significant

目的:探讨上皮性屏障分子E-钙黏蛋白和闭锁蛋白在鼻黏膜上皮中的表达情况和意义。方法:纳入对照组下鼻甲黏膜13例,嗜酸性鼻息肉10例,非嗜酸性鼻息肉14例,单纯性慢性鼻窦炎11例。利用免疫组织化学方法检测屏障分子E-钙黏蛋白和闭锁蛋白在不同类型慢性鼻窦炎上皮的表达情况。结果:伴和不伴息肉的慢性鼻-鼻窦炎各组和对照组的E-钙黏蛋白表达差异无统计学意义(P>0.05),在各组中E-钙黏蛋白表达在黏膜上皮和腺体上皮。而闭锁蛋白主要表达在上皮细胞顶端的最外侧,在对照组表达连续,而在慢性鼻窦炎各组上皮细胞顶端闭锁蛋白的表达出现中断、不连续的现象,表达下调。结论:上皮屏障分子E-钙黏蛋白和闭锁蛋白在不同类型慢性鼻-鼻窦炎中的表达情况,提示在鼻黏膜慢性炎症过程中,细胞间黏附分子的表达无明显缺失,而主要紧密连接蛋白在慢性炎症的黏膜上皮表达下调,在上皮细胞的顶端表达中断,引起上皮的屏障功能异常,因而病原微生物更容易侵入,引起鼻黏膜不同程度的炎症,这可能为鼻窦炎发病的机制之一。

Objective：To explore the expression and distribution of barrier molecules in epithelium of various types of chronic rhinosinusitis and its significance.Method：There were four groups including control（13 samples）,Eos-CRSwNP（10 samples）,nonEos-CRSwNP（14 samples）,CRSsNP（11 samples）.The method of immunohistochemistry was used to detect the protein expression of E-cadherin and occludin in nasal mucosa.Result：There was positive staining extensively distributed among cells in nasal mucosa.There was no significant difference in these groups.However,the occludin mainly located on the top of epithelial cells.In normal nasal mucosa,the positive expression was continuous,however,it was discontinuous both in CRSwNP and CRSsNP groups.Conclusion：There was no E-cadherin loss in the progression of pathophysiology of chronic rhinosinusitis.But the loss of occludin may correlate to the dysfunction of epithelial barrier,which was beneficial for the pathogen invasion.