复方丹参滴丸对I/R大鼠心脏表面血流量和心肌损伤的改善作用

Pre-treatment with Cardiotonic Pills Attenuates Ischemia-reperfusion-induced Myocardial Blood Flow and Myocardial Injury in Rats

目的:探讨复方丹参滴丸(CP)预给药对缺血30min再灌注24h后大鼠心肌损伤的改善作用。方法:雄性SD大鼠60只,分为模型组(12只)、假手术组(12只)、给药(0.1g/Kg、0.4g/Kg、0.8g/Kg)组(各12只),结扎模型组大鼠冠状动脉左前降支30min,解除结扎再灌注24h,建立缺血再灌注(I/R)模型。假手术组只穿线不结扎。给药各组在手术前90min分别一次性灌胃给予CP(0.1g/Kg、0.4g/Kg和0.8g/Kg)后,行模型组相同手术和操作。各组大鼠均采用激光多普勒血流灌注成像仪测定心脏表面血流量;TTC染色法测定心肌梗死面积;TUNEL染色法检测心肌凋亡细胞。结果:模型组大鼠心脏表面血流量明显降低、心肌梗死面积增加、凋亡心肌细胞数增多。三个CP剂量组都能增加心脏表面血流量、减少心肌梗死面积、抑制心肌细胞凋亡。结论:CP具有改善I/R24h大鼠心脏血流量和心肌损伤的作用。

Objective：To investigate the effect of cardiotonic pills（CP） on myocardial damage during ischemia-reperfusion（I/R） injury.Method：60 Sprague-Dawley（SD） rats were used and divided into 5 groups for evaluating various parameters of concern.Myocardial I/R model was induced by occlusion left anterior descending coronary artery for 30 min and reperfusion for 24 hours,which were subjected to I/R with or without pre-treatment with CP（0.1 g/Kg,0.4 g/Kg or 0.8 g/Kg）.Myocardial blood flow（MBF） was monitored by using the Laser Doppler imaging technique.Tthe infarct size was detected by TTC staining,and the apoptotic cells were analyzed by TUNEL staining.Results：Pre-treatment with CP significantly alleviated I/R induced disorders in myocardial microvasculature,manifested as the reduction of MBF.CP also decreased myocardial infarct size and myocardial apoptotic cells at 24 h after reperfusion.Conclusion：Pre-treatment with CP could attenuate I/R-induced myocardial blood flow and myocardial injury.