尼古丁对人脐静脉内皮细胞凋亡及坏死的影响

Effect of nicotine on human umbilical vein endothelial cells

目的不同浓度尼古丁对人脐静脉内皮细胞(HUVECs)凋亡及坏死的影响。方法用CD34以免疫组织化学法鉴定HUVECs。通过3种不同浓度的尼古丁(3、30、300 ng/ml)刺激HUVECs 24 h后,用流式细胞仪检测其凋亡及坏死率。结果低浓度尼古丁促进细胞凋亡最强,而随着尼古丁浓度的增加,细胞凋亡率反而逐渐降低,各组差异具有统计学意义(P<0.05);此外,随着尼古丁浓度增加,细胞坏死率也日益增多,各组差异具有统计学意义(P<0.05)。细胞坏死率与尼古丁浓度呈正相关(r=0.675)。结论尼古丁对HUVECs凋亡的影响具有浓度依赖性,细胞坏死率与尼古丁浓度呈正相关。

Objective To investigate the effect of nicotine on the mode of human umbilical vein endothelial cells（HUVECs） death.Methods CD34 were used as a marker to identify HUVECs by immunocytochemistry.HUVECs were treated with three different concentrations of nicotine（3,30 and 300 ng/ml） for 24 hours.The rate of apoptosis or necrosis was analyzed with Flow cytometry.Results A low concentration of nicotine（3 ng/ml） was found to promote cell apoptosis.The percentage of apoptosis was significantly decreased when the concentration of nicotine was increased（P0.05）.The reduction of apoptosis was accompanied with an increased in necrosis.The increase in necrosis was positively correlated with an increasing concentration of nicotine（r=0.675）.Conclusion Nicotine dose-dependently increases necrosis of human umbilical vein endothelial cells.