卵巢移植对去势大鼠海马神经元形态及BDNF表达的影响

Ovarian transplantation on playing morphological changes and BDNF expression in hippocampal neurons of castrated rat

目的:观察卵巢移植后大鼠海马神经元形态变化及BDNF的表达,评价卵巢移植对海马神经元的保护效果。方法:制作去势SD大鼠模型,卵巢移植干预组在其肾被膜下移植出生3 d内的SD乳鼠卵巢,外源性激素干预组隔天给予腹腔注射乙烯雌酚1 mg/kg。分别于干预后的4、7、14和28 d,电镜观察海马神经元形态及免疫组化观察BDNF的表达情况。结果:卵巢移植后随着移植时间的延长血清雌激素和孕酮水平都逐步升高,乙烯雌酚组仅雌激素水平升高;各组海马神经元形态逐渐好转,移植14 d和28 d组海马神经元的形态改善优于同期给药组。卵巢移植组和乙烯雌酚组BDNF的表达在各区均有所升高,卵巢移植14 d和28 d组CA3区尤为明显(P<0.05)。结论:卵巢移植可以恢复卵巢内分泌功能,对海马神经元形态有改善作用及促进BDNF的表达,其神经元保护作用优于单纯乙烯雌酚干预组。

Objective：To observe the impact of ovarian transplantation on playing morphological changes and BDNF expression in rat hippocampal neurons,evaluation of ovarian transplantation on the protective effect in hippocampal neurons.Methods： The model groups were established by female SD rats were used for ovariectomy（OVX）.One intervention group was transplanted the ovaries of SD rat within 3 days under the kidney capsule.Another group was injected diethylstilbestro（1 mg/kg） intraperitoneally every other day.To observe hippocampal neuronal morphology by the electron microscopy and expression of BDNF by immunohistochemical method after the ovarian transplantation of the 4,7,14 and 28 days.Results： With the migration time,the serum estrogen and progesterone levels have gradually increased in ovarian transplantation group,while only estrogen have increased in diethylstilbestrol group.Morphology of hippocampal neurons in each group gradually improved,the ovarian transplantation group was significantly increased compared with diethylstilbestrol group,especially in 14 d and 28 d.Expression of BDNF in each group have all increased at every regions in the hippocampus,and the changes of transplantation group is remarkable in CA3 area in 14 d and 28 d（P0.05）.Conclusion： Ovarian transplantation can restore ovarian endocrine function,improve the morphology of hippocampal neurons and promote the expression of BDNF.The neuroprotective role in the transplantation group was better than diethylstilbestrol group.