STAT3、VEGF蛋白在涎腺腺样囊性癌中的表达及对血管生成的影响

The Expression of STAT3 and VEGF in Salivary Adenoid Cystic Carcinoma and Its Effect on Angiogenesis

目的:探讨信号转导和转录活化因子-3(STAT3)及血管内皮生长因子(VEGF)蛋白在涎腺腺样囊性癌(SACC)组织中的表达情况及其与SACC临床病理参数、血管生成的关系。方法:采用免疫组织化学法检测50例SACC(SACC组)与15例正常腮腺组织(对照组)中STAT3、VEGF蛋白表达和微血管密度(MVD)。结果:(1)SACC组的STAT3及VEGF蛋白表达阳性率分别为66%和72%,明显高于正常组的6.7%和20%(P<0.01)。(2)STAT3和VEGF的表达在SACC的不同组织类型、TNM分期、有无淋巴结转移方面差异均有统计学意义(P<0.05),但在不同性别、年龄及肿瘤部位方面差异均无统计学意义(P>0.05)。(3)STAT3与VEGF表达呈正相关(P<0.05);SACC组的STAT3蛋白及VEGF蛋白表达中阳性与阴性者的MVD差异比较均有统计学意义(均P<0.05)。结论:STAT3可能通过上调VEGF表达,促进SACC中新生血管的形成,STAT3可能会成为SACC的抗血管生成的治疗靶点。

Objective： To investigate expressions of signal transducer and transcription 3 （STAT3） and vascular endothe- lial growth factor （VEGF） in salivary adenoid cystic carcinoma, and explore its relation to elinieopathologieal parameters and tumor angiogenesis. Methods： Expressions of STAT3 and VEGF and values of mierovessel density were detected by immuno- histochemistry in samples of tumor tissues from 50 patients with salivary adenoid cystic carcinoma （SACC group） and those of normal parotid gland tissues from 15 healthy individuals （control group）. Results： （1）The positive expression rates of STAT3 and VEGF were 66% and 72% in SACC group, which were significantly higher than those of control group （6.7% and 20%, P 〈 0.01）. （2） Expressions of STAT3 and VEGF protein were significantly correlated with SACC clinical pathological type, lymph node metastasis and TNM stage （P 〈 0.05）, but no correlation with gender, age and the location of tumor （P 〉 0.05）. （3） There was a positive correlation between STAT3 and VEGF in salivary adenoid cystic carcinoma （P 〈 0.05）. In 50 specimens of salivary adenoid cystic carcinoma, there was a significant difference in MVD between positive expression and negative ex- pression of STAT3 and VEGF sub-groups in SACC group （P 〈 0.05）. Conclusion： By raising VEGF, STAT3 might promote the formation of new blood vessels in SACC. STAT3 pathway can be a new way to prevent angiogenesis of SACC.