不同浓度臭氧水对口腔综合治疗台水道系统中乙肝病毒作用的研究

Study on Effects of Different Concentrations of Ozone Solution on the HBV Virus in Dental Unit Waterlines

目的:研究不同质量浓度臭氧溶液对口腔综合治疗台水道系统(DUWLs)中乙肝病毒(HBV)的灭活作用,探讨限时灭活HBV的最低臭氧溶液浓度。方法:常规培养2215细胞,在室温5℃、震荡混合、密闭等条件下配制不同浓度的臭氧溶液。将臭氧溶液加入2215细胞培养瓶中,作用不同时间后,取其上清液,提取HBV-DNA,聚合酶链式反应(PCR)扩增,荧光定量(FQ-PCR)检测HBV-DNA。结果:水温、密闭条件对臭氧溶液的质量浓度有影响,在上述条件下配置的臭氧溶液浓度稳定,臭氧溶液能抑制2215细胞表达HBV-DNA,臭氧浓度越高,其对HBV-DNA的灭活作用越强。臭氧浓度为0.6mg/L10min及0.5mg/L15min能将设定浓度的HBV-DNA灭活。结论:0.5～0.6mg/L臭氧水溶液可用于杀灭口腔综合治疗台水系统中的HBV。

Objective： To study effects of different concentrations of ozone solution on the hepatitis B virus （HBV） in dental unit waterlines （DUWLs）, and find out the lowest ozone water concentration to achieve maximum HIV viral inactiva- tion. Methods： The human 2215 cells were cultured. Under the room temperature 5℃, concussion mixed and airtight condi- tions, different concentrations of aqueous solution of ozone were prepared. The ozone solution was added into the 2215 culture flask. Different concentrations of aqueous solution of ozone acted with the supernatant liquid of the human 2215 ceils in differ- ent time points. The HBV-DNA was extracted from the 2215 cell supernatant. Following fluorescence quantitative PCR ampli- fication, HBV-DNA was detected by FQ-PCR. Results： It was found that water temperature and airtight affected the mass concentration of ozone solution. Under those conditions, the aqueous solution of ozone was stable. The higher the ozone con- centration, the stronger the inactivation of HBV-DNA expression in 2215 cells. In activation of HBV-DNA was found in the ozone concentration of 0.6 mg/L for 10 minutes and the ozone concentration of 0.5 mg/L for 15 min. Conclusion： The ozone concentration of 0.5-0.6 mg/L can be used to inactivate HBV in DUWLs.