斑秃患者皮损丝聚蛋白的表达

Expression of filaggrin in alopecia areata lesions of patients

目的探讨丝聚蛋白表达水平与斑秃患者特应性素质和疾病严重程度的关系。方法分析37例斑秃患者的临床资料及实验室资料，取斑秃皮损和正常对照的头皮进行免疫组化染色，用荧光半定量RT—PCR检测22例斑秃皮损和正常对照的头皮丝聚蛋白在蛋白质和信使RNA的表达水平。结果斑秃皮损丝聚蛋白和其mRNA的表达水平较正常对照明显降低（P〈0．05或0．01），而且这种降低在脱发面积较大、病程较长和有指甲改变的斑秃患者中更明显，但降低水平与是否伴发特应性疾病无关。斑秃患者伴特应性疾病组与不伴特应性疾病组之间性别、发病年龄、病程、脱发面积、家族史、甲改变、血清IgE和嗜酸粒细胞升高的发生率等方面差异均无统计学意义。结论斑秃患者其皮损丝聚蛋白及其mRNA的表达水平均降低，提示丝聚蛋白可能参与斑秃的发病，并和疾病的严重程度有关。

Objective To assess the relationship of filaggrin expression with atopic diathesis and disease severity in patients with alopeeia areata （AA）. Methods Thirty-seven patients with AA aged （26.3 ± 10.6） years were enrolled in this study. Atopie diseases were noted in 8 of these patients. Clinical data and laboratory test results were reviewed. Immunohistoehemieal staining was performed to quantify the expression of filaggrin protein in scalp biopsy specimens from all of the 37 patients with AA and from 10 human controls, and fluorescence-based semiquantitative reverse transeription-PCR to detect the expression of filaggrin mRNA in scalp biopsy specimens from 22 patients with AA and 13 healthy controls . Data were statistically analyzed by Mann Whitney U test, ehi-square test, and Spearman＇s rank correlation test. Results The expressions of filaggTin protein and mRNA were significantly lower in patients with AA than in the controls （P 〈 0.05 or 0.01 ）, and the decrease seemed more obvious in patients with large areas of lesions, long duration of disease, and nail abnormalities, but the degree of decrease was unrelated to the complication with atopie diseases. No significant differences were observed in sex ratio, age at onset, disease duration, area of hair loss, the prevalence of family history or incidence of nail abnormalities and increase in serum IgE and eosinophils, between patients with atopie diseases and those without. Conclusions The expressions of filaggrin protein and mRNA are decreased in patients with AA, suggesting that filaggrin may participate in the development of AA and is correlated with the severity of AA.