特应性皮炎患儿外周血Th17细胞与CD4＋CD25＋ T细胞失衡的研究

Imbalance between peripheral blood Th17 cells and CD4＋CD25＋ regulatory T cells in children with atopic dermatitis

目的 探讨Th17细胞和Treg细胞失衡在特应性皮炎（AD）发病机制中的作用。方法 流式细胞仪检测52例AD患者外周血Th17细胞和Treg细胞的频率、酶联免疫吸附方法（ELISA） 检测外周血中细胞因子IL-6、TGF-β1的表达水平。同时以30例性别、年龄匹配的健康体检者作为对照。结果 AD组外周血Th17细胞（CD3＋CD8-IL17＋）占CD3＋T细胞的百分比为（1.20 ± 0.41）%，高于对照组的（0.54 ± 0.28）% （t = 2.58，P 〈 0.05）；Treg（CD4＋ CD25＋）细胞的百分比为（2.29 ± 0.67）%，低于对照组（5.95 ± 0.45）%，（t = 15.23，P 〈 0.01）。关键调控因子测定结果：IL-6水平，AD组（5.12 ± 0.45）ng/L高于对照组（3.89 ± 0.38） ng/L，差异具有统计学意义（t = 2.59，P 〈 0.05）；而TGF-β1的表达水平AD组（57.65 ± 10.78） ng/L低于对照组的（81.18 ± 7.78） ng/L，（t = 5.41，P 〈 0.01）。 结论 特应性皮炎患儿外周血Th17、Treg细胞水平及其关键的调控平衡因子IL-6、TGF-β1发生变化，其比例的失平衡可能参与特应性皮炎的发病。

Objective To evaluate the roles of imbalance between peripheral blood T helper 17 （Th17） cells and CD4＋CD25＋ regulatory T （Treg） cells in the pathogenesis of atopic dermatitis（AD）. Methods Peripheral blood samples were obtained from 52 patients with AD aged 2-14 years and 30 age- and sex-matched healthy controls. Flow cytometry was performed to detect the percentage of Th17 cells and Treg cells in peripheral blood. Meanwhile, enzyme linked immunosorbent assay （ELISA） was carried out to detect the serum level of interleukin （IL）-6 and transforming growth factor （TGF）-β1. Results The children with AD showed a higher percentage of Th17 cells but a lower percentage of Treg cells in CD3＋ T cells compared with the controls （（1.20 ± 0.41）% vs. （0.54 ± 0.28）%, t = 2.58, P 〈 0.05; （2.29 ± 0.67）% vs. （5.95 ± 0.45）%, t = 15.23, P 〈 0.01）. Moreover, the serum level of IL-6 was significantly higher, while that of TGF-β1 was lower in patients with AD than in the controls （（5.12 ± 0.45） ng/L vs. （3.89 ± 0.38） ng/L, t = 2.59, P 〈 0.05; （57.65 ± 10.78） ng/L vs. （81.18 ± 7.78） ng/L, t = 5.41, P 〈 0.01）. Conclusions Children with AD experience a change in the percentage of Thl7 cells and Treg cells in peripheral blood as well as in the serum level of IL-6 and TGF-β1, and the imbalance between Thl7 cells and Treg cells in peripheral blood may contribute to the development of AD.