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脊髓损伤后反应性星形胶质细胞的分离培养及多能性鉴定 被引量：3

Separation and culturing of reactive astrocytes in injured spinal cord and identification of their pluripotency
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摘要 目的探讨脊髓损伤(SCI)后反应性星形胶质细胞(RAS)的多能性,为其对SCI的临床治疗提供理论依据。方法成年大鼠SCI后,分离培养脊髓内RAS;对分离培养的细胞以及诱导分化后细胞行细胞免疫荧光鉴定;在培养RAS的培养基中添加5-溴脱氧尿嘧啶核苷(BrdU),观察分离培养的细胞的增殖情况。结果分离培养的细胞可同时表达胶质纤维酸性蛋白(GFAP)和巢蛋白(nestin),诱导分化细胞可表达微球相关蛋白2(MAP-2)、受体相互作用蛋白(RIP)和GFAP,培养基中添加BrdU培养一段时间后细胞球可表达BrdU。结论体内分离培养的成体大鼠SCI后的RAS具有多能性及自我更新潜能,可能作为内源性神经干细胞修复损伤脊髓。 Objective To explore the pluripotency of reactive astrocytes （RAS） in spinal cord injury （SCI） and provide the theory evidence for clinical treatment of SCI. Methods RAS were separated and cultured from adult rats SCI. Cellular immunofluoreseence was used to identify these cells and the induced differentiation cells. The BrdU was added to the medium of RAS in order to analysis the proliferation of the cell spheres. Results The cells separated and cultured in vivo could express both GFAP and nestin. The induced differentiation cells could express MAP-2, GFAP and RIP. With BrdU added to the medium for culturing several days, the cell spheres could express BrdU. Conclusion RAS which separated and cultured form adult rats SCI has the potential of pluripotency and self-renew, and might be the endogenous neural stem cells to cure SCI.
作者 沈政 尹宗生 高维陆 张辉
机构地区 安徽医科大学第一附属医院骨科
出处 《安徽医科大学学报》 CAS 北大核心 2012年第6期638-641,共4页 Acta Universitatis Medicinalis Anhui
基金 国家自然科学基金(编号:30973026) 安徽省自然科学基金(编号:11040606Q25)
关键词 脊髓损伤 反应性星形胶质细胞 内源性神经干细胞 巢蛋白 spinal cord injury reactive astrocytes endogenous neural stem cells nestin
分类号 R681.54 [医药卫生—骨科学]
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参考文献9

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同被引文献56

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引证文献3

二级引证文献7

安徽医科大学学报
2012年 第6期

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