心律失常与心肌梗死患者红细胞异常的光镊拉曼光谱分析

Study of abnormal erythrocyte in patients with arrhythmia and myocardial infarction by optical tweezers Raman spectroscopy

目的利用激光光镊拉曼光谱系统,测定健康者、心律失常患者和心肌梗死患者红细胞的拉曼光谱。方法取新鲜静脉血样本,经洗涤、离心处理后分离出红细胞,用激光光镊拉曼系统采集其红细胞的拉曼光谱。结果与正常红细胞比较,心血管疾病患者红细胞的光镊拉曼光谱有较大的差异,红细胞的整体谱线偏弱;部分特征谱数发生位移。结论通过对红细胞的分离和捕获并测定其拉曼散射光谱,研究和分析红细胞的内容物及生物大分子的变化。进一步收集并建立心血管疾病的拉曼光谱数据库,为分子水平上探讨常见心血管疾病的类型及发病机制提供依据。

Objective To measure the red blood cells＇ Raman spectra of healthy person, patients of arrhythmia and myocardial infarction with laser optical tweezers Raman spectroscopy system. Methods Taking the fresh venous blood samples,then isolating red blood cells after washed and centrifuged, and determining Raman spectra of red blood cells by optical tweezers Raman spectroscopy system. Results Compared with normal red blood cells, the overall intensity of Raman spectra in patients with cardiovascular disease was significantly different. The overall spectrum of red blood cells was weaker. Some characteristic peaks had a few displacements. Conclusion According to measure the Raman scattering spectrum through separating and capturing red blood cells. Researching and analyzing the contents of red blood cell and the changes of biological macromolecules through quick and easy spectral analysis methods, so as to further collect the Raman spectra data of cardiovascular disease, and provide theoretical and experimental basis for studying the type and pathogenesis of common cardiovascular disease at the molecu- lar level.