白细胞介素-8基因781C/T多态性与迟发性阿尔茨海默病遗传易感性的关系研究

The relationship study between interleukin-8 gene polymorphism and genetic susceptibility of late-onset Alzheimer's disease

目的探讨白细胞介素-8(IL-8)基因781C/T多态性与新疆汉族迟发性阿尔茨海默病(LOAD)遗传易感性的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测80例LOAD患者与80例正常人IL-8基因781C/T多态性分布。结果①在IL-8基因781C/T基因型频率分布:LOAD组CC、CT、TT基因型分别为26.3%、38.8%、34.9%,对照组CC、CT、TT基因型分别为26.3%、43.6%、30.1%,两组比较差异无统计学意义(χ2=0.550,P=0.760);②LOAD组C、T等位基因频率分别为45.6%、54.4%,对照组C、T分别为48.1%、51.9%,两组比较差异无统计学意义(χ2=0.201,P=0.654)。结论 IL-8基因781C/T多态性可能与LOAD易感性无关。

Objective To explore the relationship between interleukin-8 （IL-8） gene 781C/T polymorphism and late-onset Alzheimer＇ s disease （LOAD）. Methods Polymorphism distribution of IL-8 gene 781C/T in 80 cases LOAD and 80 normal controls were detected by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）analysis. Results （1） Genotype frequencies of IL-8 gene 781 C/T： CC, CT and TT genotypes in LOAD group were 26. 3%, 38.8% and 34. 9%, respectively, they were 26.3%, 43.6% and 30. 1% in the control group respectively, comparison with two groups, there were significant difference （ X2 = 0. 550, P = 0. 760）. （2） Allele frequencies of IL-8 gene 781 C/T,in LOAD group C and T allele frequencies were 45.6% and 54.4% , and in control group C and T were 48. 1% and 51.9% respectively, comparison with two groups, there were significant difference （X2 = 0. 201, P = 0. 654）. Conclusion IL-8 gene 781 C/T polymorphism probably has no relation with LOAD.