新异养硝化菌Colloides sp. JZ1-1的鉴定及其硝化性能

Identification of a new heterotrophic nitrobacterium strain Colloides sp. JZ1-1 and its nitrifying capability

从驯化后的活性污泥中筛分、诱变出一株性能较好的异养硝化菌JZ1-1.经形态及生理生化特性分析,鉴定菌株JZ1-1为胶样菌属(Colloidessp.).分别考察了碳源、C/N、pH、溶解氧、温度和铵态氮初始浓度对JZ1-1硝化性能的影响.结果表明:菌株对柠檬酸钠的利用较好;C/N为10～14、30℃、pH6-9和转速150r·min-1以上有利于铵态氮的降解;菌株对中高浓度铵态氮废水(100mg·L-1≤铵态氮浓度≤500mg·L-1)的降解效果显著.经5次继代培养,菌株的稳定性较好.

A heterotrophic nitrobacterium strain JZI-1 with higher nitrifying capability was isolated and mutagenized from an acclimated activated sludge. The JZI-1 was identified as Colloides sp. , according to its morphological and physiological features. The factors affecting the nitrifying capabil- ity of JZI-1 were investigated, including medium carbon source, C/N ratio, pH value, dissolved oxygen, temperature, and ammonium nitrogen concentration. The optimal carbon source was sodium citrate, and the nitrification was favored when the C/N ratio was from 10 to 14, temperature was 30 ℃, and pH value was 6-9 when cultured at a rotating speed of more than 150 r ·min-1. JZI-1 could degrade ammonium nitrogen effectively when the initial concentration of ammonium nitrogen was from 100 mg · L-1 to 500 mg @ L. JZ1-1 was stable after 5 generations of subculture.